High-resolution liquid chromatography of DNA fragments on non-porous poly(styrene-divinylbenzene) particles

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High-resolution liquid chromatography of DNA fragments on non-porous poly(styrene-divinylbenzene) particles.

DNA restriction fragments and PCR products were separated by means of ion-pair reversed-phase high-performance liquid chromatography on alkylated non-porous poly(styrene-divinylbenzene) particles with a mean diameter of 2.1 microns. Optimum resolution was obtained by using an acetonitrile gradient in 100 mM of triethylammonium acetate and a column temperature of 50 degrees C. This allowed the s...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1993

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/21.5.1061